1. 首页 > 资源库 > 中文 > 植物所论文库

Mutation of Rice BC12/GDD1, Which Encodes a Kinesin-Like Protein That Binds to a GA Biosynthesis Gene Promoter,Leads to Dwarfism with Impaired Cell Elongation

作　　者：Li J,Jiang JF,Qian Q,Xu YY,Zhang C,Xiao J,Du C,Luo W,Zou GX,Chen ML,Huang YQ, Feng YQ, Cheng ZK,Yuan M and Chong K
影响因子：9.293
刊物名称：The Plant Cell
出版年份：2011
卷：  期：  页码：DOI: 10.1105/tpc.110.081901

论文摘要：

 The kinesins are a family of microtubule-based motor proteins that move directionally along microtubules and are involved in many crucial cellular processes, including cell elongation in plants. Less is known about kinesins directly regulating gene transcription to affect cellular physiological processes. Here, we describe a rice (Oryza sativa) mutant, gibberellin-deficient dwarf1 (gdd1), that has a phenotype of greatly reduced length of root, stems, spikes, and seeds. This reduced length is due to decreased cell elongation and can be rescued by exogenous gibberellic acid (GA3) treatment. GDD1 was cloned by a map-based approach, was expressed constitutively, and was found to encode the kinesin-like protein BRITTLE CULM12 (BC12). Microtubule cosedimentation assays revealed that BC12/GDD1 bound to microtubules in an ATP-dependent manner. Whole-genome microarray analysis revealed the expression of ent-kaurene oxidase (KO2), which encodes an enzyme involved in GA biosynthesis, was downregulated in gdd1. Electrophoretic mobility shift and chromatin immunoprecipitation assays revealed that GDD1 bound to the element ACCAACTTGAA in the KO2 promoter. In addition, GDD1 was shown to have transactivation activity. The level of endogenous GAs was reduced in gdd1, and the reorganization of cortical microtubules was altered. Therefore, BC12/GDD1, a kinesin-like protein with transcription regulation activity, mediates cell elongation by regulating the GA biosynthesis pathway in rice.
全文链接：
版权所有 © 中国科学院植物研究所　备案号：京ICP备16067583号-24　文保网安备案号：1101080078
地址：北京市海淀区香山南辛村20号　邮编：100093
电话：010-62590835