报告题目：Protein distribution system in plant cells and its contribution to control ABA homeostasis in plant cells
报告时间：2010年4月12日 16:00
报告地点：图资楼多功能厅
报告人：Inhwan Hwang
联系人：金京波研究员（jinjb@ibcas.ac.cn）
报告人简介：Professor Inhwan Hwang joined the Department of Life Science at POSTECH as an associate professor in 1999 from Gyeongsang National University where he taught for six years. He got his postdoctoral training at the Genetics Department of Harvard Medical School from 1988 to 1993 right after obtaining his doctoral degree in 1988 at the University of North Carolina-Chapel Hill. He earned his BS and MS degrees at Seoul National University in 1981 and 1983, respectively. Now he is the Director of the Creative Research Center for Plant Intracellular trafficking.
Abstract
In the eukaryotic cell such as plant cells, cellular activities are divided into various subcellular compartments called organelles. These organelles perform specific functions that depend on organelle-specific proteins. However, these organelles do not have or have very limited capacity to synthesize their constituent proteins. Accordingly, most of organellar proteins are synthesized in the cytosol and subsequently imported into these organelles. These organellar proteins are distributed by two different modes, protein targeting and protein trafficking. Proteins destined to the nucleus, ER, chloroplasts and mitochondria are targeted to their destination directly from the cytosol. In contrast, proteins of the Golgi apparatus, endosomes, lytic vacuole, protein storage vacuole and plasma membrane will be transported by vesicle trafficking after initial targeting to the ER. I will talk about our findings on the mechanism of protein targeting to chloroplasts. In addition, I will talk about how protein trafficking can control ABA homeostasis in plant cells and contribute to the osmotic stress responses in Arabidopsis. For the protein targeting, my talk will focus on the role of AKR2 and Hsc70-4. In the ABA homeostasis, I will focus on the role of AtBG1 in the ER and AtBG2 in the vacuole both of which are involved in production of ABA by hydrolyzing ABA-GE to ABA.
代表性论文：
Lee DW, Kim JK, Lee S, Choi S, Kim S, Hwang I. (2008) Arabidopsis nuclear-encoded plastid transit peptides contain multiple sequence subgroups with distinctive chloroplast-targeting sequence motifs.
Plant Cell. 20(6):1603-22
Bae WS, Lee YJ, Kim DH, Lee JH, Kim S, Sohn EJ, Hwang I. (2008) AKR2A-mediated import of chloroplast outer membrane proteins is essential for chloroplast biogenesis. Nat Cell Biol. 10(2):220-227
Lee KH, Piao HL, Kim HY, Choi SM, Jiang F, Hartung W, Hwang I, Kwak JM, Lee IJ, Hwang I. (2006) Activation of Glucosidase via Stress-Induced Polymerization Rapidly Increases Active Pools of Abscisic Acid. Cell. 126:1109-1120
Song J, Lee MH, Lee GJ, Yoo CM, Hwang I. (2006) Arabidopsis EPSIN1 Plays an Important Role in Vacuolar Trafficking of Soluble Cargo Proteins in Plant Cells via Interactions with Clathrin, AP-1, VTI11, and VSR1. Plant Cell. 18:2258-2274
Kim H, Park M, Kim SJ, Hwang I. (2005) Actin filaments play a critical role in vacuolar trafficking at the Golgi complex in plant cells. Plant Cell. 17(3):888-902
Jin JB, Bae H, Kim SJ, Jin YH, Goh CH, Kim DH, Lee YJ, Tse YC, Jiang L, Hwang I. (2003).
Arabidopsis dynamin-like proteins, ADL1C and ADL1E, play a critical role in mitochondrial morphogenesis. Plant Cell. 15:2357-2369.
Sohn EJ, Kim ES, Zhao M, Kim SJ, Kim H, Kim YW, Lee YJ, Hillmer S, Sohn U, Jiang L, Hwang I. (2003) Rha1, an Arabidopsis Rab5 homolog, plays a critical role in the vacuolar trafficking of soluble cargo proteins. Plant Cell. 15:1057-1070.
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