题　 目：Metabolic engineering of the phenylpropanoid pathway to discover novel functions of these compounds in plants

报告人：Dr. Oliver Yu

(Danforth Plant Science Center, St. Louis, Missouri. USA)

时间：2010年4月12日上午10：00-12：00

地点：图资楼217会议室

联系人：舒庆艳 (shuqy@ibcas.ac.cn)

报告人简介：Dr. Oliver Yu ，1991年毕业于上海复旦大学，1991-1992 在中科院植物所工作，于1998年在南卡罗来纳大学(Univ. of South Carolina-Columbia, SC) 获得博士学位。1998-2001在杜邦公司作物遗传学部做博士后。2002年至今在密苏里Danforth 植物科学中心工作，任副教授兼首席科学家，同时兼任华盛顿大学生物系和密苏里大学植物科学系副教授。主要从事苯丙氨酸代谢途径调控和解析，尤其是在异黄酮代谢途径调控方面具有很深的造诣。论文曾经发表在《Nature Biotechnology》，《Genome Biology》，《JACS》,《Trends in Biotechnology》，《Trends in Plant Science》，《The Plant Journal》等杂志上。 通过蛋白质组学、分子生物学和植物化学等手段研究苯丙氨酸代谢途径中次生代谢物如类黄酮与环境胁迫、大豆、苜蓿固氮作用之间关系，以及通过对异黄酮代谢途径的调控，改良豆科植物、单子叶植物及不合成异黄酮的双子叶植物的营养品质。到目前为止，发表了40余篇SCI收录的高水平专业论文。已主持并完成美国国家级课题20项，目前在研并主持课题9项。作为导师指导博士后、博士生、访问学者等35余人次。2000年以来被各大学院校、研究机构、企业等单位邀请进行学术报告30余次。现为 JBC 杂志和 GM Crop 杂志的审委, 并作为很多知名期刊如 Plant Cell, Plant Journal, Plant Physiology等的审稿人和基金委员会（NFS, USDA）的评委。

发表文章：

Gutierrez-Gonzalez JJ, Guttikonda SK, Aldrich D, Tran PLS, Zhong R, Yu O, Nguyen HT, Sleper DA (2010) Differential expression of isoflavone biosynthetic genes in soybean during water stresses. Plant and Cell Physiology, Submitted.

Gutierrez-Gonzalez JJ, Wu X, Gillman JD, Lee JD, Zhong R, Shannon JG, Yu O, Nguyen HT, Sleper DA (2010) Intricate environment-modulated genetic networks control isoflavone accumulation in soybean seeds. BMC Plant Biology. Accepted.

Yu O, Wang Y (2009) The functions of flavonoids during legume-rhizobia interactions. In Ecological Aspects of Nitrogen Metabolism in Plants. Joe Polacco and Chris Todd (eds). Accepted.

Yamaguchi M, Valliyodan B, Zhang J, Lenoble ME, Yu O, Rogers EE, Nguyen HT, Sharp RE (2009) Regulation of growth response to water stress in the soybean primary root. I. Proteomic analysis reveals region-specific regulation of phenylpropanoid metabolism and control of free iron in the elongation zone. Plant Cell Environment. 33:223-243.

Subramanian S, Cho U-H, Keyes CA, Yu O (2009) Proteomic analysis of soybean xylem sap in response to symbiotic and pathogenic interactions. BMC Plant Biology 2009, 9:119.(http://www.biomedcentral.com/1471-2229/9/119 ).

Gutierrez-Gonzalez JJ, Wu X, Zhang J, Lee JD, Ellersieck M, Shannon GJ, Yu O, Nguyen HT, Sleper DA (2009) Genetic control of soybean seed isoflavone content: Importance of statistical model and epistasis in complex traits. Theoretical and Applied Genetics 119: 1069-1083.

Keyes CA, Subramanian S, Yu O (2009) Hairy root as a model system for undergraduate laboratory curriculum and research. Bioscene 35: 6-12.

Zhang J, Yu O (2008) Metabolic engineering of isoflavone biosynthesis in seeds. In Modification of seed composition to promote health and nutrition. Hari Krishnan (ed). Agronomy Monograph Series, pp 151-177.

Zhang J, Subramanian S, Stacey G, Yu O (2008) Flavones and flavonols play distinct critical roles during nodulation of Medicago truncatula by Sinorhizobium meliloti. The Plant Journal, 57: 171-183.

Cheng H, Yu O, Yu D (2008) Polymorphisms of IFS1 and IFS2 genes are associated with isoflavone concentrations in soybean seeds. Plant Science, 175: 505-512.

Graham TL, Graham MY, Yu O (2008) Genomics of secondary metabolism in soybean. In Genetics and Genomics of Soybean. Gary Stacey (ed.), Springer, New York. pp. 211-242.

Yu O, Jez JM (2008) Nature’s assembly line: Biosynthesis of simple phenylpropanoids and polyketides. Plant Journal 54: 750-762.

Li L, He H, Zhang J, Wang X, Bai S, Stolc V,Tongprasit W, Young ND, Yu O, Deng XW (2008) Transcriptional analysis of highly syntenic regions between Medicago truncatula and Glycine max using tiling microarrays. Genome Biology, 9: R57. (http://genomebiology.com/2008/9/3/R57).

Subramanian S, Fu Y, Sunkar R, Barbazuk WB, Zhu JK, Yu O (2008) Novel and nodulationregulated microRNAs in soybean roots. BMC Genomics 9: 160 (http://www.biomedcentral.com/1471-2164/9/160). Highly accessed article.

Schroeder AC, Kumaran S, Hicks LM, Cahoon RE, Halls C, Yu O, Jez JM (2008) Contributions of conserved serine and tyrosine residues to catalysis, ligand binding, and cofactor processing in the active site of tyrosine ammonia lyase. Phytochemistry 69: 1496-1506.

Halls, C, Yu O (2008) Potential for metabolic engineering of resveratrol biosynthesis. Trends in Biotechnology, 26:77-81.

Zhang J, Subramanian S, Zhang Y, Yu O (2007) Flavone synthases from Medicago truncatula are flavanone-2-hydroxylases and are important for nodulation. Plant Physiology 144: 741-751

Graham TL, Graham MY, Subramanian S, Yu O (2007) RNAi silencing of genes for elicitation or biosynthesis of 5-deoxyisoflavonoids suppresses race-specific resistance and HR cell death in Phytophthora sojae infected tissues. Plant Physiology 144: 724-740.

Subramanian S, Stacey G, Yu O (2007) Distinct, critical roles of flavonoids during determinate and indeterminate legume nodulation. Trends in Plant Science 12: 282-285.

Chabaud M, Boisson-Dernier A, Zhang J, Taylor CG, Yu O, Barker DG (2006) Agrobacterium rhizogenes-mediated root transformation. In The Medicago truncatula handbook. Ulrike Mathesius (ed), http://www.noble.org/MedicagoHandbook/.

Subramanian S., Stacey G., Yu O (2006) Endogenous isoflavones are essential for soybean- Bradyrhizobium japonicum interactions. Plant Journal 48: 261-273.

Zhang Y, Liu ZH, Jia L, Peng ZL, Jaworski J, Wang XM, Jez J., Chen F, Yu O (2006) Using unnatural protein fusions to engineer resveratrol biosynthesis in yeast and mammalian cells. Journal of American Chemistry Society. 128: 13030-13031. Featured in Chemical and Engineering News, 84 (Oct. 2, 2006): page 43.

Ralston L, Yu O. (2006) Metabolons involving plant cytochrome P450s. Phytochemistry Review. 5: 459-472.

Yu O. (2006) Metabolic engineering of the plant phenylpropanoid pathway. Encyclopedia of Plant and Crop Science, Dekker Publishing. DOI: 10.1081/E-EPCS-120010589.

Yu O, Matsuno M, Subramanian S. (2006) Flavonoids in flowers: Genetics and Biochemistry. In Floriculture, ornamental and plant biotechnology: Advances and topical issues (1st Edition), Jaime A Teixeira da Silva (ed.), pp. 283-293.

Ralston L, Subramanian S, Matsuno M, Yu O. (2005) Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast (Saccharomyces cerevisiae) using soybean type I and type II chalcone isomerases. Plant Physiology 137: 1375-1388.

Subramanian S, Graham ML, Yu O, Graham T. (2005) Silencing of soybean isoflavone synthase through an RNAi approach leads to silencing in non-transformed tissue and to enhanced susceptibility to Phytophthora sojae. Plant Physiology 137: 1345-1353.

Yu O, McGonigle B. (2005) Metabolic engineering of isoflavone biosynthesis. Advances in Agronomy 86: 147-189.

Bennett JO, Yu O, Heartherly LG, Krishnan HB. (2004). Accumulation of genistein and daidzein, soybean isoflavones implicated in promoting human health, is significantly elevated by irrigation. Journal of Agricultural and Food Chemistry 52: 7574-7579.

Subramanian S, Xu L, Lu G, Odell J, Yu O. (2004) The promoters of the isoflavone synthase genes respond differentially to nodulation and defense signals in transgenic soybean roots. Plant Molecular Biology 54:226-239.

Yu O, Shi J, Hession A, Maxwell C, McGonigle B, Odell J. (2003) Metabolic engineering to increase isoflavone biosynthesis in soybean seed. Phytochemistry 63: 753-763

Odell J., Yu O (2001) A method for altering the isoflavonoid profile in the seed of an isoflavonoidproducing plant. US Patent No. 60/311461 (filed in June 2001), and 20060218672 (filed in Sept. 2006).

Odell J. Yu O, Hu X, G Lu (2001) Root-specific, stimulant inducible promoter and its use. US Patent No. 7,196,247B2 (Issued: Mar. 27, 2007).

Yu O, Jung W, Shi J, Crose R, Fader G, McGonigle B, Odell J. (2000) Production of the Isoflavones genistein and daidzein in non-legume dicot and monocot tissues. Isoflavone accumulation is related to activity of the phenylpropanoid pathway. Plant Physiology 124: 781-793

Jung W, Yu O, Lau CSM, O’keefe DP, Odell J, Fader G, McGonigle B. (2000) Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes. Nature Biotechnology 18: 208-212.

Fader G, Jung W, McGonigle B, Odell J, Yu O. (1999) Nucleic acid fragments encoding isoflavone synthease. WO 00/44909, US Patent No 7,098,011B1 (Issued: Aug. 29, 2006).

Yu X, Sukumarian S, Marton L. (1998) Differential expression of the Arabidopsis Nia1 and Nia2genes. Plant Physiology 116: 1091-1096.

Czako M, Wilson J, Yu X, Marton L. (1993) Sustained root culture for generation and vegetative propagation of transgenic Arabidopsis thaliana. Plant Cell Reports 12: 603-606.